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immunocult nk cell base medium  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc immunocult nk cell base medium
    <t>NK</t> <t>cell</t> characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.
    Immunocult Nk Cell Base Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunocult+nk+cell+base+medium/magnetic+beads/pmc12025879-41-56-62
    Average 90 stars, based on 1 article reviews
    immunocult nk cell base medium - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles"

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles

    Journal: Journal of Extracellular Biology

    doi: 10.1002/jex2.70049

    NK cell characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.
    Figure Legend Snippet: NK cell characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.

    Techniques Used: Purification, Flow Cytometry, Control, Expressing

    TEM images of purified EVs derived from different serum‐free NK cell culture media. (A) TEM images of EVs obtained from static culture conditions. (B) TEM images of EVs obtained from shaking culture conditions. Scale bar 200 nm. Images are representative of 8–10 images from each sample.
    Figure Legend Snippet: TEM images of purified EVs derived from different serum‐free NK cell culture media. (A) TEM images of EVs obtained from static culture conditions. (B) TEM images of EVs obtained from shaking culture conditions. Scale bar 200 nm. Images are representative of 8–10 images from each sample.

    Techniques Used: Purification, Derivative Assay, Cell Culture

    Related Articles

    Recombinant:

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles
    Article Snippet: .. NK‐92 cells (5 × 10 7 cells) were resuspended in 65 mL of serum‐free media with 10 ng/mL of recombinant human IL‐15 (R&D Systems) and cultured for 48 h. Six different serum‐free media optimized for culturing of NK cells were tested: CellGenix GMP SCGM (xeno‐free, Sartorius [abbreviated GMP SCGM]), CTS NK‐Xpander Medium (xeno‐free, ThermoFisher [abbreviated CTS]), Immunocult NK Cell Base Medium (xeno‐free, StemCell Technologies, abbreviated Immunocult), ExCellerate Human NK Cell Expansion Media, Xeno‐Free (R&D Systems, abbreviated ExCellerate XF), and ExCellerate Human NK Cell Expansion Media, Animal Component Free (R&D Systems, abbreviated ExCellerate ACF). .. Additionally, AIM V (ThermoFisher) which is formulated for serum‐free culture of lymphocytes and has been previously used in our research, was used.

    Cell Culture:

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles
    Article Snippet: .. NK‐92 cells (5 × 10 7 cells) were resuspended in 65 mL of serum‐free media with 10 ng/mL of recombinant human IL‐15 (R&D Systems) and cultured for 48 h. Six different serum‐free media optimized for culturing of NK cells were tested: CellGenix GMP SCGM (xeno‐free, Sartorius [abbreviated GMP SCGM]), CTS NK‐Xpander Medium (xeno‐free, ThermoFisher [abbreviated CTS]), Immunocult NK Cell Base Medium (xeno‐free, StemCell Technologies, abbreviated Immunocult), ExCellerate Human NK Cell Expansion Media, Xeno‐Free (R&D Systems, abbreviated ExCellerate XF), and ExCellerate Human NK Cell Expansion Media, Animal Component Free (R&D Systems, abbreviated ExCellerate ACF). .. Additionally, AIM V (ThermoFisher) which is formulated for serum‐free culture of lymphocytes and has been previously used in our research, was used.

    Article Title: Mechano-induced homotypic patterned domain formation by monocytes
    Article Snippet: B cells were cultured in ImmunoCult-XF B cell base medium (STEMCELL) with B cell expansion supplement (STEMCELL). .. NK cells were cultured in ImmunoCult NK cell base medium (STEMCELL) with NK cell expansion supplement (STEMCELL). .. T cells were cultured in X-VIVO 15 medium (Lonza) with 10% v/v FBS (Corning), IL-2 (R&D) and CD3/CD28 T cell activator (STEMCELL).



    Similar Products

    immunocult nk cell base medium  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc immunocult nk cell base medium
    <t>NK</t> <t>cell</t> characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.
    Immunocult Nk Cell Base Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunocult+nk+cell+base+medium/magnetic+beads/pmc12025879-41-56-62
    Average 90 stars, based on 1 article reviews
    immunocult nk cell base medium - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    NK cell characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.

    Journal: Journal of Extracellular Biology

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles

    doi: 10.1002/jex2.70049

    Figure Lengend Snippet: NK cell characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.

    Article Snippet: NK‐92 cells (5 × 10 7 cells) were resuspended in 65 mL of serum‐free media with 10 ng/mL of recombinant human IL‐15 (R&D Systems) and cultured for 48 h. Six different serum‐free media optimized for culturing of NK cells were tested: CellGenix GMP SCGM (xeno‐free, Sartorius [abbreviated GMP SCGM]), CTS NK‐Xpander Medium (xeno‐free, ThermoFisher [abbreviated CTS]), Immunocult NK Cell Base Medium (xeno‐free, StemCell Technologies, abbreviated Immunocult), ExCellerate Human NK Cell Expansion Media, Xeno‐Free (R&D Systems, abbreviated ExCellerate XF), and ExCellerate Human NK Cell Expansion Media, Animal Component Free (R&D Systems, abbreviated ExCellerate ACF).

    Techniques: Purification, Flow Cytometry, Control, Expressing

    TEM images of purified EVs derived from different serum‐free NK cell culture media. (A) TEM images of EVs obtained from static culture conditions. (B) TEM images of EVs obtained from shaking culture conditions. Scale bar 200 nm. Images are representative of 8–10 images from each sample.

    Journal: Journal of Extracellular Biology

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles

    doi: 10.1002/jex2.70049

    Figure Lengend Snippet: TEM images of purified EVs derived from different serum‐free NK cell culture media. (A) TEM images of EVs obtained from static culture conditions. (B) TEM images of EVs obtained from shaking culture conditions. Scale bar 200 nm. Images are representative of 8–10 images from each sample.

    Article Snippet: NK‐92 cells (5 × 10 7 cells) were resuspended in 65 mL of serum‐free media with 10 ng/mL of recombinant human IL‐15 (R&D Systems) and cultured for 48 h. Six different serum‐free media optimized for culturing of NK cells were tested: CellGenix GMP SCGM (xeno‐free, Sartorius [abbreviated GMP SCGM]), CTS NK‐Xpander Medium (xeno‐free, ThermoFisher [abbreviated CTS]), Immunocult NK Cell Base Medium (xeno‐free, StemCell Technologies, abbreviated Immunocult), ExCellerate Human NK Cell Expansion Media, Xeno‐Free (R&D Systems, abbreviated ExCellerate XF), and ExCellerate Human NK Cell Expansion Media, Animal Component Free (R&D Systems, abbreviated ExCellerate ACF).

    Techniques: Purification, Derivative Assay, Cell Culture